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Project objectives

The BIOTTOPE project is designed to improve the quality of WWTP effluents, to reach a “good status” for coastal and inland waters, based on biological effects. The principal technological innovation of this project will be the full scale reading flow system to evaluate endocrine disruptor of wastewater by using small model amphibian and fish models.


The BIOTTOPE project aims to validate an innovative wastewater treatment system combining biological processes, biological monitoring and tailored physicalchemical technologies to generate effluents free from pollutants causing biological effects. Work on the project will be based at two test sites, Brussels (Belgium) and Saint Thibault des Vignes (France).


The project will implement, test and validate a new prototype water treatment technology at semi-industrial scale, based on activated carbon adsorption and on an accelerated settling step to remove micro-pollutants such as EDCs that are not removed by the conventional treatment systems. It aims to demonstrate the technical feasibility of new operational parameters and combination technologies without decreased performance in COD (chemical oxygen demand) and BOD5 (biochemical oxygen demand in five days) reduction.


Treatment efficiency will be monitored using small biological aquatic organisms - tadpoles or fish larvae. These will be genetically modified so that they are fluorescent when they are in contact with specific EDCs. This will enable the project to monitor and enhance the performance of the combined wastewater treatment systems.